Title  
From Fold to Surface: A Spatial Atlas of Archaeal Protein Processing and Cleavage Biochemistry (v2.0)  
  
Summary  
This dataset maps archaeal protein maturation across space and mechanism, linking:  
targeting route -> folding module -> cleavage/processing enzyme -> post-processing destination.  
  
Focus  
- Cytosolic folding via archaeal proteostasis systems (including thermosome-centered pathways)  
- N-terminal signal peptide processing during Sec/Tat-associated export  
- Prepilin-like N-terminal processing for appendage biogenesis  
- C-terminal processing and surface anchoring associated with archaeosortase pathways  
  
What is new in v2.0  
- Spatially explicit fields (cellular_zone, processing_site_context)  
- Cleavage polarity normalization (N-terminus, C-terminus, Internal, None)  
- Standardized evidence + confidence + curation status  
- Integrated system rows for pathway-level interpretation  
  
Files  
- archaeal_fold_surface_atlas_v2.csv (main dataset)  
- data_dictionary.csv (field definitions and allowed values)  
- citation_map.csv (reference keys and APA citations)  
- method.txt (curation protocol and QC)  
- CHANGELOG.txt (version history)  
- LICENSE.txt (reuse terms)  
  
Intended use  
Educational and research communication, mechanistic synthesis, concept mapping, and hypothesis framing.  
  
Not intended for  
Clinical diagnosis, treatment decisions, or guideline substitution.  
  
Suggested citation  
Zhao, K. (2026). From Fold to Surface: A Spatial Atlas of Archaeal Protein Processing and Cleavage Biochemistry (v2.0) [Data set]. Harvard Dataverse.  
  
Keywords  
archaea; proteostasis; thermosome; signal peptidase; prepilin peptidase; archaeosortase; protein maturation; surface anchoring; spatial biochemistry  
